验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, human kidney tissue, Jurkat cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver tissue, human breast cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12423-1-AP targets SLC20A1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 | SLC20A1 fusion protein Ag3084 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 20 (phosphate transporter), member 1 |
| 别名 | PIT1, Phosphate transporter 1, Leukemia virus receptor 1 homolog, GLVR-1, GLVR1 |
| 计算分子量 | 679 aa, 74 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC019944 |
| 基因名称 | SLC20A1 |
| Gene ID (NCBI) | 6574 |
| RRID | AB_2286212 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WUM9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC20A1 (also known as PiT1) is a high-affinity sodium-dependent phosphate (Pi) import protein. It is widely expressed and plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions. SLC20A1 is involved in cell proliferation, tumor growth and cell signaling independently of its transport function. SLC20A1 also functions as a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC20A1 antibody 12423-1-AP | Download protocol |
| IHC protocol for SLC20A1 antibody 12423-1-AP | Download protocol |
| IP protocol for SLC20A1 antibody 12423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cancer GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity | ||
Adv Healthc Mater Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds
| ||
J Biol Chem Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake. |
