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验证数据展示

  • WB analysis using 31069-1-AP

    WB analysis using 31069-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31069-1-AP (SLC22A10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 31069-1-AP

    IHC staining of mouse liver using 31069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31069-1-AP (SLC22A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 31069-1-AP

    IHC staining of mouse liver using 31069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31069-1-AP (SLC22A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC22A10 Polyclonal antibody

SLC22A10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31069-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

hOAT5, OAT5, Organic anion transporter 5, SLC22A10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, PC-12 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31069-1-AP targets SLC22A10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SLC22A10 fusion protein Ag34521 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 22, member 10
Synonyms hOAT5, OAT5, Organic anion transporter 5, SLC22A10
Calculated Molecular Weight541 aa, 60 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_001039752
Gene Symbol SLC22A10
Gene ID (NCBI) 387775
RRIDAB_3669842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ63ZE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Organic anion transporter 5 (OAT5), also known as SLC22A10, belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of endogenous and exogenous organic ions (PMID: 20865662). Rare variants in SLC22A10 may be associated with Alzheimer's disease CSF profile of neuronal injury and inflammation (PMID: 35173266).

实验方案

Product Specific Protocols
WB protocol for SLC22A10 antibody 31069-1-APDownload protocol
IHC protocol for SLC22A10 antibody 31069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols