验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, PC-12 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31069-1-AP targets SLC22A10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | SLC22A10 fusion protein Ag34521 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | solute carrier family 22, member 10 |
Synonyms | hOAT5, OAT5, Organic anion transporter 5, SLC22A10 |
Calculated Molecular Weight | 541 aa, 60 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | NM_001039752 |
Gene Symbol | SLC22A10 |
Gene ID (NCBI) | 387775 |
RRID | AB_3669842 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | Q63ZE4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Organic anion transporter 5 (OAT5), also known as SLC22A10, belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of endogenous and exogenous organic ions (PMID: 20865662). Rare variants in SLC22A10 may be associated with Alzheimer's disease CSF profile of neuronal injury and inflammation (PMID: 35173266).
实验方案
Product Specific Protocols | |
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WB protocol for SLC22A10 antibody 31069-1-AP | Download protocol |
IHC protocol for SLC22A10 antibody 31069-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |