验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, PC-3 cells |
Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26833-1-AP targets SLC22A16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | SLC22A16 fusion protein Ag25270 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | solute carrier family 22 (organic cation/carnitine transporter), member 16 |
Synonyms | Carnitine transporter 2, CT2, Flipt 2, FLIPT2, OCT6, OKB1, SLC22A16 |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC047565 |
Gene Symbol | SLC22A16 |
Gene ID (NCBI) | 85413 |
RRID | AB_2923576 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86VW1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC22A16, also named as CT2, FLIPT2, or OCT6, belongs to the major facilitator (TC 2.A.1) superfamily and organic cation transporter (TC 2.A.1.19) family. SLC22A16 encodes an organic zwitterion transporter protein that transports carnitine and some anticancer drugs, including anthracyclines. As a carnitine transporter, SLC22A16 is involved in the regulation of fatty acid oxidation (FAO), which plays an important role also in the metabolic transformation of cancer cells. SLC22A16 expression in cancer cells is associated with increased sensitivity to the cytotoxic effects of doxorubicin. SLC22A16 has 3 isoforms with the molecular mass of 58, 61 and 65 kDa (PMID: 35761855, 35608746).
实验方案
Product Specific Protocols | |
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WB protocol for SLC22A16 antibody 26833-1-AP | Download protocol |
IHC protocol for SLC22A16 antibody 26833-1-AP | Download protocol |
IF protocol for SLC22A16 antibody 26833-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |