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验证数据展示

  • IHC staining of mouse brain using 24901-1-AP

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24901-1-AP

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of L02 using 24901-1-AP

    WB analysis of L02 using 24901-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 24901-1-AP

    IP experiment of mouse liver using 24901-1-AP

    IP result of anti-SLC22A23 (IP:24901-1-AP, 4ug; Detection:24901-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

SLC22A23 Polyclonal antibody

SLC22A23 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24901-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

BOCT2, C6orf85, SLC22A23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24901-1-AP targets SLC22A23 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC22A23 fusion protein Ag19036 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 22, member 23
别名 BOCT2, C6orf85, SLC22A23
计算分子量 686 aa, 74 kDa
观测分子量 66-74 kDa
GenBank蛋白编号BC128580
基因名称 SLC22A23
Gene ID (NCBI) 63027
RRIDAB_2879787
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA1A5C7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC22A23 antibody 24901-1-APDownload protocol
IHC protocol for SLC22A23 antibody 24901-1-APDownload protocol
IP protocol for SLC22A23 antibody 24901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols