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SLC22A23 Polyclonal antibody

SLC22A23 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24901-1-AP

Print datasheet

Synonyms

BOCT2, C6orf85, SLC22A23

验证数据展示

  • IHC staining of mouse brain using 24901-1-AP

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24901-1-AP

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of L02 using 24901-1-AP

    WB analysis of L02 using 24901-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 24901-1-AP

    IP experiment of mouse liver using 24901-1-AP

    IP result of anti-SLC22A23 (IP:24901-1-AP, 4ug; Detection:24901-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24901-1-AP targets SLC22A23 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC22A23 fusion protein Ag19036 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 22, member 23
Synonyms BOCT2, C6orf85, SLC22A23
Calculated Molecular Weight 686 aa, 74 kDa
Observed Molecular Weight 66-74 kDa
GenBank Accession NumberBC128580
Gene Symbol SLC22A23
Gene ID (NCBI) 63027
RRIDAB_2879787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA1A5C7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC22A23 antibody 24901-1-APDownload protocol
IHC protocol for SLC22A23 antibody 24901-1-APDownload protocol
IP protocol for SLC22A23 antibody 24901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols