验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, 37°C incubated mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13238-1-AP targets SLC22A4 in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | SLC22A4 fusion protein Ag3974 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 22 (organic cation/ergothioneine transporter), member 4 |
| 别名 | Ergothioneine transporter, ET transporter, ETT, OCTN1, SLC22A4, UT2H |
| 计算分子量 | 551 aa, 62 kDa |
| 观测分子量 | 62-70 kDa |
| GenBank蛋白编号 | BC028313 |
| 基因名称 | SLC22A4 |
| Gene ID (NCBI) | 6583 |
| RRID | AB_3669155 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H015 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC22A4 (solute carrier family 22 member 4), also known as OCTN1. It is expected to be located in cell membrane and mitochondrion membrane. It is strongly expressed in kidney, trachea, bone marrow and fetal liver and in several human cancer cell lines, but not in adult liver (PMID: 9426230). The protein functions as a Na+-dependent and pH-dependent high affinity microbial symporter of potent food-derived antioxidant ergothioeine (PubMed:15795384), which transports one sodium ion with one ergothioeine molecule. The calculated molecular weight of SLC22A4 is 62 kDa, the band of 70 kDa is glycosylated (PMID:26724204).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC22A4 antibody 13238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |