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验证数据展示

  • WB analysis of SMMC-7721 using 67479-1-Ig

    WB analysis of SMMC-7721 using 67479-1-Ig

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67479-1-Ig

    IHC staining of human liver cancer using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included
  • IHC staining of human liver cancer using 67479-1-Ig

    IHC staining of human liver cancer using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included
  • IHC staining of human liver using 67479-1-Ig

    IHC staining of human liver using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included
  • IHC staining of mouse liver using 67479-1-Ig

    IHC staining of mouse liver using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included

SLC22A7 Monoclonal antibody

SLC22A7 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67479-1-Ig

产品说明书

CloneNo. 3D3C3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

SLC22A7, 3D3C3, hOAT2, NLT, OAT2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SLC22A7 Ready-To-Use IHC Kit. SLC22A7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67479-1-Ig targets SLC22A7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 SLC22A7 fusion protein Ag25234 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 22 (organic anion transporter), member 7
别名 SLC22A7, 3D3C3, hOAT2, NLT, OAT2
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC033805
基因名称 SLC22A7
Gene ID (NCBI) 10864
RRIDAB_2882706
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y694
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Organic anion transporter (OAT)2 (SLC22A7) belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of numerous organic ions including xenobiotics and endogenous substrates (PMID: 9529348, 20190416). SLC22A7 was originally identified as a novel liver-specific transporter because of its predominant mRNA expression in the rat liver (PMID: 15900017, 25904762). Human SLC22A7 exhibits a robust transport function for a wide array of naturally occurring nucleobases, nucleosides, and nucleotides with a particular role for cGMP (PMID: 18216183).

实验方案

Product Specific Protocols
WB protocol for SLC22A7 antibody 67479-1-IgDownload protocol
IHC protocol for SLC22A7 antibody 67479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols