验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, A431 cells, Jurkat cells |
Positive IHC detected in | mouse brain tissue, human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
67467-1-Ig targets SLC25A12 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | Human, Mouse |
免疫原 | SLC25A12 fusion protein Ag13576 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | solute carrier family 25 (mitochondrial carrier, Aralar), member 12 |
别名 | AGC1, ARALAR, ARALAR1, SLC25A12 |
计算分子量 | 75 kDa, 63 kDa |
观测分子量 | 70-75 kDa |
GenBank蛋白编号 | BC016932 |
基因名称 | SLC25A12 |
Gene ID (NCBI) | 8604 |
RRID | AB_2882696 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | O75746 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SLC25A12 antibody 67467-1-Ig | Download protocol |
IHC protocol for SLC25A12 antibody 67467-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |