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SLC25A12 Monoclonal antibody

SLC25A12 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2B10

Cat no : 67467-1-Ig

Print datasheet

Synonyms

AGC1, ARALAR, ARALAR1, SLC25A12

验证数据展示

  • WB analysis of LNCaP using 67467-1-Ig

    WB analysis of LNCaP using 67467-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67467-1-Ig

    IHC staining of mouse brain using 67467-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, A431 cells, Jurkat cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67467-1-Ig targets SLC25A12 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen SLC25A12 fusion protein Ag13576 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms AGC1, ARALAR, ARALAR1, SLC25A12
Calculated Molecular Weight 75 kDa, 63 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC016932
Gene Symbol SLC25A12
Gene ID (NCBI) 8604
RRIDAB_2882696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75746
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC25A12 antibody 67467-1-IgDownload protocol
IHC protocol for SLC25A12 antibody 67467-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols