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验证数据展示

  • WB analysis using 10789-1-AP

    WB analysis using 10789-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 10789-1-AP

    IF Staining of U2OS using 10789-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC25A13 antibody (10789-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IHC staining of human colon cancer using 10789-1-AP

    IHC staining of human colon cancer using 10789-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10789-1-AP

    IHC staining of human colon cancer using 10789-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse kidney using 10789-1-AP

    IP experiment of mouse kidney using 10789-1-AP

    IP result of anti-SLC25A13 (IP:10789-1-AP, 4ug; Detection:10789-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

SLC25A13 Polyclonal antibody

SLC25A13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10789-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Mitochondrial aspartate glutamate carrier 2, Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial, Citrin, Calcium-binding mitochondrial carrier protein Aralar2, ARALAR-related gene 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, A431 cells, HEK-293 cells, mouse kidney tissue, mouse liver tissue, Raji cells, mouse ovary tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10789-1-AP targets SLC25A13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC25A13 fusion protein Ag1242 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25, member 13 (citrin)
别名 Mitochondrial aspartate glutamate carrier 2, Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial, Citrin, Calcium-binding mitochondrial carrier protein Aralar2, ARALAR-related gene 2
计算分子量 74 kDa
观测分子量 60-63 kDa
GenBank蛋白编号BC006566
基因名称 SLC25A13
Gene ID (NCBI) 10165
RRIDAB_2189738
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJS0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A13, also known as citrin, has been identified as a mitochondrial aspartate-glutamate carrier protein and may be implicated in metabolic compartmentation. It consists of 675 amino acid residues and harbors four EF-hands and six mitochondrial transmembranous (TM) spanners. Mutations in SLC25A13 gene cause adult-onset type II citrullinemia (CTLN2) and neonatal intrahepatic cholestasis (NICCD). The complete deletion or cleaved SLA25A13 proteins have been reported in patients with SLC25A13 mutation.

实验方案

Product Specific Protocols
WB protocol for SLC25A13 antibody 10789-1-APDownload protocol
IHC protocol for SLC25A13 antibody 10789-1-APDownload protocol
IF protocol for SLC25A13 antibody 10789-1-APDownload protocol
IP protocol for SLC25A13 antibody 10789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols