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验证数据展示

  • WB analysis of RAW264.7 using 21568-1-AP

    WB analysis of RAW264.7 using 21568-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 21568-1-AP (SLC25A25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21568-1-AP

    IHC staining of human liver using 21568-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21568-1-AP (SLC25A25 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 21568-1-AP

    IHC staining of human liver using 21568-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21568-1-AP (SLC25A25 antibody) at dilution of 1:50 (under 40x lens).

SLC25A25 Polyclonal antibody

SLC25A25 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21568-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

APC3, KIAA1896, MCSC, MCSC3, PCSCL, RP11 395P17.4, SCAMC 2, SCAMC2, SLC25A25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21568-1-AP targets SLC25A25 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 SLC25A25 fusion protein Ag16086 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25
别名 APC3, KIAA1896, MCSC, MCSC3, PCSCL, RP11 395P17.4, SCAMC 2, SCAMC2, SLC25A25
计算分子量 469 aa, 53 kDa
观测分子量 50 kDa, 100 kDa
GenBank蛋白编号BC103933
基因名称 SLC25A25
Gene ID (NCBI) 114789
RRIDAB_10858638
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6KCM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC25A25 antibody 21568-1-APDownload protocol
IHC protocol for SLC25A25 antibody 21568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Transcriptome-wide analysis of PGC-1α-binding RNAs identifies genes linked to glucagon metabolic action.

Authors - Clint D J Tavares
View Article