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SLC25A25 Polyclonal antibody

SLC25A25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21568-1-AP

Print datasheet

Synonyms

APC3, KIAA1896, MCSC, MCSC3, PCSCL, RP11 395P17.4, SCAMC 2, SCAMC2, SLC25A25

验证数据展示

  • WB analysis of RAW264.7 using 21568-1-AP

    WB analysis of RAW264.7 using 21568-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 21568-1-AP (SLC25A25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21568-1-AP

    IHC staining of human liver using 21568-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21568-1-AP (SLC25A25 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 21568-1-AP

    IHC staining of human liver using 21568-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21568-1-AP (SLC25A25 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 21568-1-AP is SLC25A25 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen SLC25A25 fusion protein Ag16086 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25
Synonyms APC3, KIAA1896, MCSC, MCSC3, PCSCL, RP11 395P17.4, SCAMC 2, SCAMC2, SLC25A25
Calculated Molecular Weight 469 aa, 53 kDa
Observed Molecular Weight 50 kDa, 100 kDa
GenBank Accession NumberBC103933
Gene Symbol SLC25A25
Gene ID (NCBI) 114789
RRIDAB_10858638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6KCM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC25A25 antibody 21568-1-APDownload protocol
IHC protocol for SLC25A25 antibody 21568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Transcriptome-wide analysis of PGC-1α-binding RNAs identifies genes linked to glucagon metabolic action.

Authors - Clint D J Tavares
View Article