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  • Featured Product
  • KD/KO Validated

SLC25A32 Polyclonal antibody

SLC25A32 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13080-1-AP

Print datasheet

Synonyms

MFT, MFTC, SLC25A32

验证数据展示

  • WB analysis using 13080-1-AP

    WB analysis using 13080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13080-1-AP (SLC25A32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13080-1-AP

    IHC staining of human prostate cancer using 13080-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13080-1-AP (SLC25A32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13080-1-AP targets SLC25A32 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SLC25A32 fusion protein Ag3722 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 25, member 32
Synonyms MFT, MFTC, SLC25A32
Calculated Molecular Weight 315 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC021893
Gene Symbol SLC25A32
Gene ID (NCBI) 81034
RRIDAB_2877913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2D1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Solute carrier family 25 member 32 (SLC25A32) can transfer tetrahydrofolate (THF) from cellular cytoplasm to the mitochondria as a mitochondrial folate transporter (MFT). It has been reported that SLC25A32 can also transport FAD across the mitochondrial inner membrane. Chronic alcohol exposure affects mitochondrial folate uptake and may affect the transcription of the SLC25A32. And functional SLC25A32 defect is associated with the cranial neural tube defects(PMID: 21956163; 29666258)

实验方案

Product Specific Protocols
WB protocol for SLC25A32 antibody 13080-1-APDownload protocol
IHC protocol for SLC25A32 antibody 13080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

SLC25A32 promotes malignant progression of glioblastoma by activating PI3K-AKT signaling pathway

Authors - Zhiwei Xue
  • KD Validated
View Article