ANT1/2 Polyclonal antibody

ANT1/2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17796-1-AP

Print datasheet

Synonyms

2F1, AAC2, ADP,ATP carrier protein 2, ADP/ATP translocase 2, ANT 2, ANT1/2, SLC25A5, T2, T3



经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue, mouse heart tissue, mouse kidney tissue, human cervical cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SLC25A5 Ready-To-Use IHC Kit. SLC25A5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17796-1-AP targets ANT1/2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ANT1/2 fusion protein Ag11956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
Synonyms 2F1, AAC2, ADP,ATP carrier protein 2, ADP/ATP translocase 2, ANT 2, ANT1/2, SLC25A5, T2, T3
Calculated Molecular Weight 298 aa, 33 kDa
Observed Molecular Weight 30-32 kDa, 60 kDa
GenBank Accession NumberBC056160
Gene Symbol SLC25A5
Gene ID (NCBI) 292
RRIDAB_2190358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05141
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)

实验方案

Product Specific Protocols
WB protocol for ANT1/2 antibody 17796-1-APDownload protocol
IHC protocol for ANT1/2 antibody 17796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Elife

Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases.

Authors - Jiaqi Fu
mouseWB

PLoS Genet

Novel insights into SLC25A46-related pathologies in a genetic mouse model.

Authors - Maria Eirini Terzenidou
humanIHC

Mol Cell Proteomics

Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans.

Authors - Kathleen C Lundberg
ratWB

Exp Neurol

Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder.

Authors - Jin-Hee Seo
mouseWB

J Proteome Res

Proteomic Identification of the SLC25A46 Interactome in Transgenic Mice Expressing SLC25A46-FLAG.

Authors - Vasiliki-Iris Perivolidi
humanWB

Biology (Basel)

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.

Authors - Yanbing Wang