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验证数据展示

  • WB analysis using 51031-1-AP

    WB analysis using 51031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51031-1-AP (SLC25A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 51031-1-AP

    IHC staining of human lung cancer using 51031-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 51031-1-AP

    IHC staining of human lung cancer using 51031-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC25A6 Polyclonal antibody

SLC25A6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51031-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ANT 3, ANT 2, ADP/ATP translocase 3, N-terminally processed, ADP/ATP translocase 3, ADP,ATP carrier protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, K-562 cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

51031-1-AP targets SLC25A6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
别名 ANT 3, ANT 2, ADP/ATP translocase 3, N-terminally processed, ADP/ATP translocase 3, ADP,ATP carrier protein 3
计算分子量 33 kDa
观测分子量30-33 kDa
GenBank蛋白编号BC031912
基因名称 SLC25A6
Gene ID (NCBI) 293
RRIDAB_2190370
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12236
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).

实验方案

Product Specific Protocols
WB protocol for SLC25A6 antibody 51031-1-APDownload protocol
IHC protocol for SLC25A6 antibody 51031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Int J Oncol

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.

Authors - Mohamed E M Saeed
View Article
humanWB

MethodsX

Complete solubilization of mammalian cells in lysates

Authors - Izabela Bednarska
View Article