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验证数据展示

  • IHC staining of mouse cerebellum using 14156-1-AP

    IHC staining of mouse cerebellum using 14156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14156-1-AP (SLC26A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 14156-1-AP

    IHC staining of mouse cerebellum using 14156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14156-1-AP (SLC26A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14156-1-AP

    WB analysis using 14156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14156-1-AP (SLC26A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SLC26A11 Polyclonal antibody

SLC26A11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Sodium-independent sulfate anion transporter, kidney brain anion transporter, KBAT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, unboiled mouse brain tissue, mouse kidney tissue, unboiled mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14156-1-AP targets SLC26A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC26A11 fusion protein Ag5338 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 26, member 11
别名 Sodium-independent sulfate anion transporter, kidney brain anion transporter, KBAT
计算分子量 606 aa, 65 kDa
观测分子量65-72 kDa
GenBank蛋白编号BC035900
基因名称 SLC26A11
Gene ID (NCBI) 284129
RRIDAB_3085430
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WA9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC26A11 is a sodium-independent sulfate transporter from high endothelial venules (PMID: 12626430). SLC26A11 exhibits sodium-independent sulfate anion transporter activity that may cooperate with SLC26A2 to mediate DIDS-sensitive sulfate uptake into high endothelial venules endothelial cells (HEVEC) (PMID: 12626430). SLC26A11 is critical for inhibitory transmission and contributes to locomotor coordination in Purkinje cells (PMID: 27390771). SLC26A11, a chloride transporter, localizes with the vacuolar H+-ATPase of A-intercalated cells of the kidney and can be detected at 72kDa (PMID: 21716257).

实验方案

Product Specific Protocols
WB protocol for SLC26A11 antibody 14156-1-APDownload protocol
IHC protocol for SLC26A11 antibody 14156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols