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验证数据展示

  • IHC staining of mouse cerebellum using 14156-1-AP

    IHC staining of mouse cerebellum using 14156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14156-1-AP (SLC26A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 14156-1-AP

    IHC staining of mouse cerebellum using 14156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14156-1-AP (SLC26A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14156-1-AP

    WB analysis using 14156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14156-1-AP (SLC26A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SLC26A11 Polyclonal antibody

SLC26A11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14156-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Sodium-independent sulfate anion transporter, kidney brain anion transporter, KBAT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, unboiled mouse brain tissue, mouse kidney tissue, unboiled mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14156-1-AP targets SLC26A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC26A11 fusion protein Ag5338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 26, member 11
Synonyms Sodium-independent sulfate anion transporter, kidney brain anion transporter, KBAT
Calculated Molecular Weight 606 aa, 65 kDa
Observed Molecular Weight65-72 kDa
GenBank Accession NumberBC035900
Gene Symbol SLC26A11
Gene ID (NCBI) 284129
RRIDAB_3085430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WA9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC26A11 is a sodium-independent sulfate transporter from high endothelial venules (PMID: 12626430). SLC26A11 exhibits sodium-independent sulfate anion transporter activity that may cooperate with SLC26A2 to mediate DIDS-sensitive sulfate uptake into high endothelial venules endothelial cells (HEVEC) (PMID: 12626430). SLC26A11 is critical for inhibitory transmission and contributes to locomotor coordination in Purkinje cells (PMID: 27390771). SLC26A11, a chloride transporter, localizes with the vacuolar H+-ATPase of A-intercalated cells of the kidney and can be detected at 72kDa (PMID: 21716257).

实验方案

Product Specific Protocols
WB protocol for SLC26A11 antibody 14156-1-APDownload protocol
IHC protocol for SLC26A11 antibody 14156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols