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验证数据展示

  • WB analysis using 12776-1-AP

    WB analysis using 12776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 12776-1-AP

    IF Staining of mouse testis using 12776-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SLC26A8 Polyclonal antibody

SLC26A8 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 12776-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA and More (1)

别名

TAT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12776-1-AP targets SLC26A8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 SLC26A8 fusion protein Ag3472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 26, member 8
别名 TAT1
计算分子量 970 aa, 109 kDa
观测分子量 62, 86 and 97 kDa
GenBank蛋白编号BC025408
基因名称 SLC26A8
Gene ID (NCBI) 116369
RRIDAB_2877880
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RN1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC26A8, also named as TAT1, acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate and oxalate transport. It has been reported that SLC26A8 is a sulfate transporter specifically expressed in spermatocytes and spermatids, playing a key role in spermatogenesis. There are several isoforms of SLC26A8 and 12776-1-AP antibody detects 62, 86 and 97 kDa protein in SDS-PAGE. (PMID: 11278976; 11834742)

实验方案

Product Specific Protocols
WB protocol for SLC26A8 antibody 12776-1-APDownload protocol
IF protocol for SLC26A8 antibody 12776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

The role of intestinal immune cells and matrix metalloproteinases in inflammatory bowel disease

Authors - Kun Mei
View Article
humanIF

Asian J Androl

The role of SLC26A8 homozygous variants in male infertility and flagellum abnormalities

Authors - Bin Mao
View Article