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FATP2 Polyclonal antibody

FATP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14048-1-AP

Print datasheet

Synonyms

SLC27A2, EC:6.2.1.3, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-

验证数据展示

  • WB analysis using 14048-1-AP

    WB analysis using 14048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14048-1-AP

    WB analysis using 14048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14048-1-AP

    IP experiment of HepG2 using 14048-1-AP

    IP result of anti-FATP2 (IP:14048-1-AP, 4ug; Detection:14048-1-AP 1:500) with HepG2 cells lysate 3200ug.

  • IHC staining of human kidney using 14048-1-AP

    IHC staining of human kidney using 14048-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 14048-1-AP

    IHC staining of human kidney using 14048-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 14048-1-AP

    IHC staining of human liver cancer using 14048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 14048-1-AP

    IHC staining of human liver cancer using 14048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 14048-1-AP

    IF Staining of HepG2 using 14048-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (14048-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver, rat kidney, rat liver, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14048-1-AP targets FATP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen FATP2 fusion protein Ag5217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 27 (fatty acid transporter), member 2
Synonyms SLC27A2, EC:6.2.1.3, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-
Calculated Molecular Weight 567 aa, 65 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC057770
Gene Symbol FATP2/SLC27A2
Gene ID (NCBI) 11001
RRIDAB_2239416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14975
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.

实验方案

Product Specific Protocols
WB protocol for FATP2 antibody 14048-1-APDownload protocol
IHC protocol for FATP2 antibody 14048-1-APDownload protocol
IF protocol for FATP2 antibody 14048-1-APDownload protocol
IP protocol for FATP2 antibody 14048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hepatology

Letter to the editor: Adipose lipolysis is important for ethanol to induce fatty liver in the NIAAA murine model of chronic and binge ethanol feeding

Authors - Wenhao Luo
View Article
mouseWB

Hepatology

Adipose lipolysis is important for ethanol to induce fatty liver in the National Institute on Alcohol Abuse and Alcoholism murine model of chronic and binge ethanol feeding.

Authors - Mallika Mathur
View Article
ratWB

Environ Pollut

Hepatotoxicity induced in rats by chronic exposure to F-53B, an emerging replacement of perfluorooctane sulfonate (PFOS)

Authors - Xiaohan Li
View Article
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
View Article
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article
mouseWB

Food Funct

Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice

Authors - Rui Guo
View Article