GLUT1 Recombinant antibody
GLUT1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
6F14
验证数据展示
经过测试的应用
Positive WB detected in | unboiled HEK-293 cells, HeLa cells (incubated at 37°C) |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
For optimal WB detection with 81463-1-RR, we recommend to avoid boiling the sample after lysis.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
81463-1-RR targets GLUT1 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | GLUT1 fusion protein Ag16282 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | solute carrier family 2 (facilitated glucose transporter), member 1 |
Synonyms | SLC2A1, SLC2A1,GLUT1, GLUT-1, 6F14 |
Calculated Molecular Weight | 492 aa, 54 kDa |
Observed Molecular Weight | 45-55 kDa |
GenBank Accession Number | BC121804 |
Gene Symbol | GLUT1 |
Gene ID (NCBI) | 6513 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P11166 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GLUT1 antibody 81463-1-RR | Download protocol |
IHC protocol for GLUT1 antibody 81463-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Br J Cancer Relationship between the expression of PD-L1 and 18F-FDG uptake in pancreatic ductal adenocarcinoma | ||
Cell Death Dis Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression |