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验证数据展示

  • WB analysis using 26958-1-AP

    WB analysis using 26958-1-AP

    Boiled and unboiled mouse heart tissue were subjected to SDS PAGE followed by western blot with 26958-1-AP (SLC2A12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 26958-1-AP

    IHC staining of mouse skeletal muscle using 26958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26958-1-AP (SLC2A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 26958-1-AP

    IHC staining of mouse skeletal muscle using 26958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26958-1-AP (SLC2A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC2A12 Polyclonal antibody

SLC2A12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26958-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Glucose transporter type 12, GLUT 12, GLUT12, GLUT8, SLC2A12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, unboiled mouse heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26958-1-AP targets SLC2A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC2A12 fusion protein Ag24979 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 2 (facilitated glucose transporter), member 12
别名 Glucose transporter type 12, GLUT 12, GLUT12, GLUT8, SLC2A12
计算分子量 617 aa, 67 kDa
观测分子量67-70 kDa
GenBank蛋白编号BC070149
基因名称 SLC2A12
Gene ID (NCBI) 154091
RRIDAB_3085917
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TD20
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC2A12 encodes glucose transporter type 12 (GLUT12), a basal and insulin-independent glucose transporter in the heart with previously reported associations with idiopathic dilated cardiomyopathy in humans, as well as insulin resistance and kidney disease in animal models (PMID: 37081215).

实验方案

Product Specific Protocols
WB protocol for SLC2A12 antibody 26958-1-APDownload protocol
IHC protocol for SLC2A12 antibody 26958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols