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SLC2A12 Polyclonal antibody

SLC2A12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26958-1-AP

Print datasheet

Synonyms

Glucose transporter type 12, GLUT 12, GLUT12, GLUT8, SLC2A12

验证数据展示

  • WB analysis using 26958-1-AP

    WB analysis using 26958-1-AP

    Boiled and unboiled mouse heart tissue were subjected to SDS PAGE followed by western blot with 26958-1-AP (SLC2A12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 26958-1-AP

    IHC staining of mouse skeletal muscle using 26958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26958-1-AP (SLC2A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 26958-1-AP

    IHC staining of mouse skeletal muscle using 26958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26958-1-AP (SLC2A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, unboiled mouse heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26958-1-AP targets SLC2A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC2A12 fusion protein Ag24979 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 2 (facilitated glucose transporter), member 12
Synonyms Glucose transporter type 12, GLUT 12, GLUT12, GLUT8, SLC2A12
Calculated Molecular Weight 617 aa, 67 kDa
Observed Molecular Weight67-70 kDa
GenBank Accession NumberBC070149
Gene Symbol SLC2A12
Gene ID (NCBI) 154091
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD20
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC2A12 encodes glucose transporter type 12 (GLUT12), a basal and insulin-independent glucose transporter in the heart with previously reported associations with idiopathic dilated cardiomyopathy in humans, as well as insulin resistance and kidney disease in animal models (PMID: 37081215).

实验方案

Product Specific Protocols
WB protocol for SLC2A12 antibody 26958-1-APDownload protocol
IHC protocol for SLC2A12 antibody 26958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols