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验证数据展示

  • WB analysis using 31429-1-AP

    WB analysis using 31429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31429-1-AP (SLC2A4RG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SLC2A4RG Polyclonal antibody

SLC2A4RG Polyclonal Antibody for WB, ELISA
Cat No. 31429-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Huntington disease gene regulatory region-binding protein 1, HDBP-1, HDBP1, HDBP 1, GLUT4 enhancer factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31429-1-AP targets SLC2A4RG in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SLC2A4RG fusion protein Ag35675 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SLC2A4 regulator
别名 Huntington disease gene regulatory region-binding protein 1, HDBP-1, HDBP1, HDBP 1, GLUT4 enhancer factor
计算分子量41kDa，387aa
观测分子量30 kDa
GenBank蛋白编号NM_020062.3
基因名称 SLC2A4RG
Gene ID (NCBI) 56731
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NR83
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC2A4RG, or SLC2A4 Regulator, is a nuclear transcription factor that plays a role in activating the solute carrier family 2 member 4 gene, also known as GLUT4. SLC2A4RG has been implicated in the transcriptional regulation of genes, including its interaction with a 7-bp consensus sequence (GCCGGCG), which is an essential cis-regulatory element for Huntington's disease (HD) gene expression in neuronal cells. The SLC2A4RG protein is involved in the modulation of SLC2A4 expression and has been identified as a transcription factor that binds specifically to the promoter region of SLC2A4. It is also known to interact with another transcription factor, myocyte enhancer factor 2 (MEF2), to activate the transcription of SLC2A4. SLC2A4RG is expressed ubiquitously in various tissues, with higher expression levels observed in fat and kidney tissues.

实验方案

Product Specific Protocols
WB protocol for SLC2A4RG antibody 31429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols