SLC30A1 Polyclonal antibody

SLC30A1 Polyclonal Antibody for WB, ELISA
Cat No. 22661-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA and More (1)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HeLa membrane protein, HepG2 total crude protein, HepG2 membrane protein

It is best to use membrane samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

22661-1-AP targets SLC30A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SLC30A1 fusion protein Ag18567 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 30 (zinc transporter), member 1
Synonyms
Calculated Molecular Weight 507 aa, 55 kDa
Observed Molecular Weight63~75 kDa
GenBank Accession NumberBC121015
Gene Symbol SLC30A1
Gene ID (NCBI) 7779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6M5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). ZnT1 is located in the plasma membrane and is responsible for Zn export from the cytosol to the extracellular space during periods of elevated cytosolic Zn. Western analysis detected a higher band at∼63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319, 15753530).

实验方案

Product Specific Protocols
WB protocol for SLC30A1 antibody 22661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Eur J Histochem

HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum.

Authors - Li Niu
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