验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17363-1-AP targets ZnT3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ZnT3 fusion protein Ag10735 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 30 (zinc transporter), member 3 |
| 别名 | SLC30A3, Zinc transporter 3, ZnT 3, ZNT3 |
| 计算分子量 | 388 aa, 42 kDa |
| 观测分子量 | 42 kDa, 48 kDa |
| GenBank蛋白编号 | BC028358 |
| 基因名称 | ZnT3 |
| Gene ID (NCBI) | 7781 |
| RRID | AB_2239335 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99726 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZnT3, encoded by SLC30A3, is a member of SLC30A family which is zinc transporter sequestrating cytosolic zinc into intracellular compartments or effluxing zinc to the extracellular space. Excessive zinc level can cause neuron apoptosis and lead to cognitive impairments. Znt3 facilitates the transport and storage of zinc in synaptic vesicles. Abnormal ZnT3 expression has been linked to some neurodegenerative disorders.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZnT3 antibody 17363-1-AP | Download protocol |
| IHC protocol for ZnT3 antibody 17363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Commun Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder. | ||
J Cereb Blood Flow Metab MicroRNA-30a regulates acute cerebral ischemia-induced blood-brain barrier damage through ZnT4/zinc pathway. | ||
Cell Biosci Disruption of zinc transporter ZnT3 transcriptional activity and synaptic vesicular zinc in the brain of Huntington's disease transgenic mouse. | ||
Int J Mol Sci MicroRNA-5572 Is a Novel MicroRNA-Regulating SLC30A3 in Sporadic Amyotrophic Lateral Sclerosis. | ||
J Neurosci Res Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis. | ||
Biochem Biophys Res Commun Protective roles of SLC30A3 against endoplasmic reticulum stress via ERK1/2 activation. |