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SLC30A8/ZNT8-Specific Polyclonal antibody

SLC30A8/ZNT8-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16169-1-AP

Print datasheet

Synonyms

SLC30A8, SLC30A8/ZNT8-Specific, Zinc transporter 8, ZnT 8, ZNT8



经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas tissue, human pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16169-1-AP targets SLC30A8/ZNT8-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, Yellow catfish
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 30 (zinc transporter), member 8
Synonyms SLC30A8, SLC30A8/ZNT8-Specific, Zinc transporter 8, ZnT 8, ZNT8
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_173851
Gene Symbol SLC30A8
Gene ID (NCBI) 169026
RRIDAB_10733889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.

实验方案

Product Specific Protocols
WB protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
IHC protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
IF protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes.

Authors - Chengfeng Merriman
ratWB

J Biol Chem

PMID: 28130446

Authors - Qiong Huang
mouseWB

J Biol Chem

A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells.

Authors - Chengfeng Merriman

J Biol Chem

Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8.

Authors - Chengfeng Merriman
humanWB

J Endocrinol

Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death.

Authors - Maria Karsai
humanIHC

Sci Rep

A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells.

Authors - Shogo Ohta