验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver cancer tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67221-1-Ig targets SLC31A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | SLC31A1 fusion protein Ag28410 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | solute carrier family 31 (copper transporters), member 1 |
别名 | Copper transporter 1, COPT1, CTR1, hCTR1, SLC31A1 |
计算分子量 | 21 kDa |
观测分子量 | 32 kDa |
GenBank蛋白编号 | BC013611 |
基因名称 | SLC31A1 |
Gene ID (NCBI) | 1317 |
RRID | AB_2882512 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O15431 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
IHC protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
IF protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
FC protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Mol Biosci Significance of cuproptosis- related genes in the diagnosis and classification of psoriasis | ||
J Virol Identification of Copper Transporter 1 as a Receptor for Feline Endogenous Retrovirus ERV-DC14.
| ||
Front Immunol Identification of hub cuproptosis related genes and immune cell infiltration characteristics in periodontitis | ||
Cell Rep A co-opted endogenous retroviral envelope promotes cell survival by controlling CTR1-mediated copper transport and homeostasis
| ||
Aging (Albany NY) Integrated bioinformatics and experiment revealed that cuproptosis is the potential common pathogenesis of three kinds of primary cardiomyopathy | ||
Cell Death Dis Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers |