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SLC31A1 Recombinant antibody

SLC31A1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240868E12

Cat no : 83844-6-RR

Print datasheet

Synonyms

COPT1, Truncated CTR1 form, CTR1, 240868E12

验证数据展示

  • IF Staining of HepG2 using 83844-6-RR

    IF Staining of HepG2 using 83844-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC31A1 antibody (83844-6-RR, Clone: 240868E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83844-6-RR

    FC experiment of HeLa using 83844-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SLC31A1 Recombinant antibody (83844-6-RR, Clone:240868E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83844-6-RR is SLC31A1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SLC31A1 fusion protein Ag26601 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 31 (copper transporters), member 1
Synonyms COPT1, Truncated CTR1 form, CTR1, 240868E12
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC013611
Gene Symbol SLC31A1
Gene ID (NCBI) 1317
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SLC31A1 antibody 83844-6-RRDownload protocol
FC protocol for SLC31A1 antibody 83844-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols