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验证数据展示

  • WB analysis using 24582-1-AP

    WB analysis using 24582-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24582-1-AP (SLC37A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 24582-1-AP

    IF Staining of MCF-7 using 24582-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC37A1 antibody (24582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 24582-1-AP

    IHC staining of human stomach cancer using 24582-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24582-1-AP (SLC37A1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC37A1 Polyclonal antibody

SLC37A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24582-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Glycerol 3 phosphate permease, Glucose-6-phosphate exchanger SLC37A1, G3PP, G-3-P permease, G 3 P transporter

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, MCF-7 cells, rat kidney tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24582-1-AP targets SLC37A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC37A1 fusion protein Ag20180 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 37 (glycerol-3-phosphate transporter), member 1
别名 Glycerol 3 phosphate permease, Glucose-6-phosphate exchanger SLC37A1, G3PP, G-3-P permease, G 3 P transporter
计算分子量 533 aa, 58 kDa
观测分子量 60-66 kDa
GenBank蛋白编号BC152997
基因名称 SLC37A1
Gene ID (NCBI) 54020
RRIDAB_2879621
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57057
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC37A1 (also known as SPX1) is one of the SLC37 family members, localized in the endoplasmic reticulum (ER) membrane (PMID:29719821). The SLC37A1 protein also shares 30% sequence identity to GlpT, suggesting that SLC37A1 could be a mammalian glycerol-3-phosphate transporter. The SLC37A1 gene is widely expressed, with the highest transcript levels in adult kidney, bone marrow, intestine, spleen, and liver (PMID:24745989). In addition, the SLC37A1 protein shares 59% homology to SLC37A2, 35% homology to SLC37A3, and 22% homology to SLC37A4 (PMID:23506893). SLC37A1 is a 533 amino-acid protein with a calculated molecular weight of 58 kDa and observed molecular weight 60-66kDa.

实验方案

Product Specific Protocols
WB protocol for SLC37A1 antibody 24582-1-APDownload protocol
IHC protocol for SLC37A1 antibody 24582-1-APDownload protocol
IF protocol for SLC37A1 antibody 24582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols