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验证数据展示

  • WB analysis of SH-SY5Y using 27636-1-AP

    WB analysis of SH-SY5Y using 27636-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27636-1-AP (SLC38A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27636-1-AP

    IHC staining of mouse brain using 27636-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27636-1-AP

    IHC staining of mouse brain using 27636-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27636-1-AP

    IF Staining of mouse brain using 27636-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC38A6 antibody (27636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC38A6 Polyclonal antibody

SLC38A6 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 27636-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

SNAT6, N-system amino acid transporter 1, NAT-1, NAT1, Amino acid transporter SLC38A6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27636-1-AP targets SLC38A6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC38A6 fusion protein Ag24654 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 38, member 6
Synonyms SNAT6, N-system amino acid transporter 1, NAT-1, NAT1, Amino acid transporter SLC38A6
Calculated Molecular Weight 456 aa, 51 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC110378
Gene Symbol SLC38A6
Gene ID (NCBI) 145389
RRIDAB_3085978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC38A6 is also known as SNAT6 and belongs to the amino acid/polyamine transporter 2 family. SLC38A6 is probably a sodium-dependent amino acid/proton antiporter or a neuronal transporter for glutamate. SLC38A6 has 2 isoforms with the molecular mass of 51 and 58 kDa. The observed molecular weight of SLC38A6 is 43 kDa, which is consistent with the description in the literature of MW between 37 and 50 kDa (PMID: 24752331).

实验方案

Product Specific Protocols
WB protocol for SLC38A6 antibody 27636-1-APDownload protocol
IHC protocol for SLC38A6 antibody 27636-1-APDownload protocol
IF protocol for SLC38A6 antibody 27636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols