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验证数据展示

  • WB analysis of SH-SY5Y using 27636-1-AP

    WB analysis of SH-SY5Y using 27636-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27636-1-AP (SLC38A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27636-1-AP

    IHC staining of mouse brain using 27636-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27636-1-AP

    IHC staining of mouse brain using 27636-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27636-1-AP

    IF Staining of mouse brain using 27636-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC38A6 antibody (27636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC38A6 Polyclonal antibody

SLC38A6 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 27636-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

SNAT6, N-system amino acid transporter 1, NAT-1, NAT1, Amino acid transporter SLC38A6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27636-1-AP targets SLC38A6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC38A6 fusion protein Ag24654 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 38, member 6
别名 SNAT6, N-system amino acid transporter 1, NAT-1, NAT1, Amino acid transporter SLC38A6
计算分子量 456 aa, 51 kDa
观测分子量43 kDa
GenBank蛋白编号BC110378
基因名称 SLC38A6
Gene ID (NCBI) 145389
RRIDAB_3085978
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC38A6 is also known as SNAT6 and belongs to the amino acid/polyamine transporter 2 family. SLC38A6 is probably a sodium-dependent amino acid/proton antiporter or a neuronal transporter for glutamate. SLC38A6 has 2 isoforms with the molecular mass of 51 kDa and 58 kDa. The observed molecular weight of SLC38A6 is 43 kDa, which is consistent with the description in the literature of MW between 37 kDa and 50 kDa (PMID: 24752331).

实验方案

Product Specific Protocols
WB protocol for SLC38A6 antibody 27636-1-APDownload protocol
IHC protocol for SLC38A6 antibody 27636-1-APDownload protocol
IF protocol for SLC38A6 antibody 27636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols