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SLC38A7 Recombinant antibody

SLC38A7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240147C3

Cat no : 83346-6-RR

Print datasheet

Synonyms

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

验证数据展示

  • WB analysis of Caco-2 using 83346-6-RR

    WB analysis of Caco-2 using 83346-6-RR

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 83346-6-RR

    WB analysis of human liver using 83346-6-RR

    human liver tissue were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of Caco-2 using 83346-6-RR

    FC experiment of Caco-2 using 83346-6-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of U-251 using 83346-6-RR

    FC experiment of U-251 using 83346-6-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83346-6-RR

    Affinity and Kinetic Characterization of 83346-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83346-6-RR against Human SLC38A7 were performed. The affinity constant is 1.31 nM.

经过测试的应用

Positive WB detected inCaco-2 cells, human liver tissue
Positive FC (Intra) detected inCaco-2 cells, U-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83346-6-RR targets SLC38A7 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SLC38A7 fusion protein Ag35081 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 38, member 7
Synonyms SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001961
Gene Symbol SLC38A7
Gene ID (NCBI) 55238
RRIDAB_3671005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NVC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC38A7 also known as SNAT7, is a member of the SLC38 family that encodes sodium-coupled neutral amino acid transporters. SLC38A7 is a system N transporter that has a substrate preference for L-glutamine. It also transports other amino acids with polar side chains, as well as L-histidine and L-alanine (PMID: 21511949).

实验方案

Product Specific Protocols
WB protocol for SLC38A7 antibody 83346-6-RRDownload protocol
FC protocol for SLC38A7 antibody 83346-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols