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SLC38A9 Recombinant antibody

SLC38A9 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240039E7

Cat No : 83182-5-RR

Print datasheet

Synonyms

URLC11, Up-regulated in lung cancer 11, Solute carrier family 38 member 9, Neutral amino acid transporter 9, 240039E7

验证数据展示

  • WB analysis of mouse pancreas using 83182-5-RR

    WB analysis of mouse pancreas using 83182-5-RR

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83182-5-RR (SLC38A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83182-5-RR

    FC experiment of U2OS using 83182-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Slc38a9 Recombinant Antibody (83182-5-RR, Clone:240039E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83182-5-RR

    Affinity and Kinetic Characterization of 83182-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83182-5-RR against Human SLC38A9 were performed. The affinity constant is 0.715 nM.

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive FC (Intra) detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83182-5-RR targets SLC38A9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC38A9 fusion protein Ag15607 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 38, member 9
Synonyms URLC11, Up-regulated in lung cancer 11, Solute carrier family 38 member 9, Neutral amino acid transporter 9, 240039E7
Calculated Molecular Weight 561 aa, 64 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC066891
Gene Symbol SLC38A9
Gene ID (NCBI) 153129
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NBW4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mTOR complex 1 (mTORC1) protein kinase is a master growth regulator that responds to multiple environmental cues. The lysosomal amino acid transporter SLC38A9 is a physical and functional component of this sensing complex. SLC38A9 (Solute Carrier Family 38 Member 9) was first identified as a potential amino acid transporter belonging to the slc38 family. SLC38A9 has some isoforms: 64,53,57 kDa.

实验方案

Product Specific Protocols
WB protocol for SLC38A9 antibody 83182-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols