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验证数据展示

  • IHC staining of human colon cancer using 21287-1-AP

    IHC staining of human colon cancer using 21287-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 21287-1-AP

    WB analysis using 21287-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21287-1-AP (SLC39A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21287-1-AP

    IHC staining of mouse brain using 21287-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 21287-1-AP

    IHC staining of human colon cancer using 21287-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 21287-1-AP

    IF Staining of HeLa using 21287-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SLC39A2 antibody (21287-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SLC39A2 Polyclonal antibody

SLC39A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21287-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ZIP-2, ZIP2, hZIP2, Eti-1, Eti 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21287-1-AP targets SLC39A2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 SLC39A2 fusion protein Ag15807 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 39 (zinc transporter), member 2
别名 ZIP-2, ZIP2, hZIP2, Eti-1, Eti 1
计算分子量 309 aa, 33 kDa
观测分子量33 kDa, 66-70 kDa
GenBank蛋白编号BC096723
基因名称 SLC39A2
Gene ID (NCBI) 29986
RRIDAB_3085650
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC39A2 (hZIP2) is a human zinc importer in the ZIP family, which is essential in the maintenance of intracellular zinc homeostasis and is upregulated by zinc depletion. SLC39A2 is highly expressed in prostate epithelial cells and is involved in prostate cancer development. Most SLC39 proteins consist of eight transmembrane domains (TMDs), with extracellular or intravesicular amino and carboxy termini (PMID: 34790705). Zip2 gene knockout exacerbated myocardial I/R injury, whereas Zip2 gene transfer reduced myocardial infarction (PMID: 31095941). The predicted molecular weight of SLC39A2 is 33 kDa, and a band of dimers at 66 kDa can also be detected by this antibody (PMID: 24619115).

实验方案

Product Specific Protocols
WB protocol for SLC39A2 antibody 21287-1-APDownload protocol
IHC protocol for SLC39A2 antibody 21287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols