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验证数据展示

  • IHC staining of human colon cancer using 21287-1-AP

    IHC staining of human colon cancer using 21287-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 21287-1-AP

    WB analysis using 21287-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21287-1-AP (SLC39A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21287-1-AP

    IHC staining of mouse brain using 21287-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 21287-1-AP

    IHC staining of human colon cancer using 21287-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 21287-1-AP

    IF Staining of HeLa using 21287-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SLC39A2 antibody (21287-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SLC39A2 Polyclonal antibody

SLC39A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21287-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ZIP-2, ZIP2, hZIP2, Eti-1, Eti 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21287-1-AP targets SLC39A2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SLC39A2 fusion protein Ag15807 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 39 (zinc transporter), member 2
Synonyms ZIP-2, ZIP2, hZIP2, Eti-1, Eti 1
Calculated Molecular Weight 309 aa, 33 kDa
Observed Molecular Weight33 kDa, 66-70 kDa
GenBank Accession NumberBC096723
Gene Symbol SLC39A2
Gene ID (NCBI) 29986
RRIDAB_3085650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC39A2 (hZIP2) is a human zinc importer in the ZIP family, which is essential in the maintenance of intracellular zinc homeostasis and is upregulated by zinc depletion. SLC39A2 is highly expressed in prostate epithelial cells and is involved in prostate cancer development. Most SLC39 proteins consist of eight transmembrane domains (TMDs), with extracellular or intravesicular amino and carboxy termini (PMID: 34790705). Zip2 gene knockout exacerbated myocardial I/R injury, whereas Zip2 gene transfer reduced myocardial infarction (PMID: 31095941). The predicted molecular weight of SLC39A2 is 33 kDa, and a band of dimers at 66 kDa can also be detected by this antibody (PMID: 24619115).

实验方案

Product Specific Protocols
WB protocol for SLC39A2 antibody 21287-1-APDownload protocol
IHC protocol for SLC39A2 antibody 21287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols