验证数据展示
经过测试的应用
| Positive WB detected in | Colo320 cells, human jejunum tissue |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20625-1-AP targets ZIP4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, hamster |
| 免疫原 | ZIP4 fusion protein Ag14524 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 39 (zinc transporter), member 4 |
| 别名 | AEZ, SLC39A4, Zinc transporter ZIP4, ZIP 4, ZIP4, Zrt and Irt like protein 4 |
| 计算分子量 | 647 aa, 68 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC062625 |
| 基因名称 | ZIP4 |
| Gene ID (NCBI) | 55630 |
| RRID | AB_10700008 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P5W5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZIP4 is a zinc transporter encoded by the SLC39A4 gene, playing an important role in zinc homeostasis in mammalian cells. Recently, many studies have indicated that ZIP4 plays important roles in many kinds of carcinoma development and progression. ZIP4 may serve as a potential diagnostic and prognostic marker for gliomas and pancreatic cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZIP4 antibody 20625-1-AP | Download protocol |
| IHC protocol for ZIP4 antibody 20625-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells. | ||
Gastroenterology Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
| ||
Clin Cancer Res ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.
| ||
Cancer Lett ZIP4 promotes non-small cell lung cancer metastasis by activating snail-N-cadherin signaling axis. | ||
Int J Mol Sci Zinc Favors Triple-Negative Breast Cancer's Microenvironment Modulation and Cell Plasticity | ||
Cancers (Basel) ZIP4 Is a Novel Cancer Stem Cell Marker in High-Grade Serous Ovarian Cancer. |
