验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, A549 cells, 4T1 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14236-1-AP targets LIV-1/ZIP6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 | LIV-1/ZIP6 fusion protein Ag5478 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 39 (zinc transporter), member 6 |
| 别名 | Ermelin, LIV 1, LIV1, LIV-1/ZIP6, SLC39A6, Zinc transporter ZIP6, ZIP 6, ZIP6, Zrt and Irt like protein 6 |
| 计算分子量 | 85 kDa |
| 观测分子量 | 85-100 kDa, 48 and 32 kDa |
| GenBank蛋白编号 | BC039498 |
| 基因名称 | SLC39A6/ZIP6 |
| Gene ID (NCBI) | 25800 |
| RRID | AB_10596924 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13433 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The zinc transporter LIV-1 (also known as SLC39A6 or ZIP6) was originally identified as an estrogen-induced gene in breast cancer cells. Elevated LIV-1 expression is reportedly related to cancer progression in various types of cancer, including breast, prostate, pancreatic, cervical and liver cancers. While correlation of LIV-1 and prognosis may vary. LIV 1 has 3 isoforms with MW of 85-100 (glycosylation and phosphorylation), 48 and 32 kDa while migrates as different bands in different lysates in SDS-PAGE.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIV-1/ZIP6 antibody 14236-1-AP | Download protocol |
| IHC protocol for LIV-1/ZIP6 antibody 14236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Genome-wide association study identifies common variants in SLC39A6 associated with length of survival in esophageal squamous-cell carcinoma.
| ||
Gastroenterology Solute Carrier Family 39 Member 6 Gene Promotes Aggressiveness of Esophageal Carcinoma Cells by Increasing Intracellular Levels of Zinc, Activating Phosphatidylinositol 3-Kinase Signaling, and Up-regulating Genes That Regulate Metastasis.
| ||
Oncotarget miR-192, a prognostic indicator, targets the SLC39A6/SNAIL pathway to reduce tumor metastasis in human hepatocellular carcinoma. | ||
Oncotarget Co-expression analysis revealed PTCH1-3'UTR promoted cell migration and invasion by activating miR-101-3p/SLC39A6 axis in non-small cell lung cancer: implicating the novel function of PTCH1.
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J Transl Med SLC39A6: a potential target for diagnosis and therapy of esophageal carcinoma. | ||
Oncol Lett Role of SLC39A6 in the development and progression of liver cancer.
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