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ZIP8 Polyclonal antibody

ZIP8 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 20459-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (3)

应用

WB, IP, IHC, ELISA and More (1)

BIGM103, PP3105, SLC39A8, ZIP 8, ZIP8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse lung tissue, mouse heart tissue, U-937 cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, mouse lung tissue, mouse small intestine tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20459-1-AP targets ZIP8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, pig, bovine
免疫原 ZIP8 fusion protein Ag14292 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 39 (zinc transporter), member 8
别名 BIGM103, PP3105, SLC39A8, ZIP 8, ZIP8
计算分子量 460 aa, 50 kDa
观测分子量 42-46 kDa, 75-90 kDa
GenBank蛋白编号BC012125
基因名称 ZIP8
Gene ID (NCBI) 64116
RRIDAB_10697830
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0K1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.

实验方案

Product Specific Protocols
WB protocol for ZIP8 antibody 20459-1-APDownload protocol
IHC protocol for ZIP8 antibody 20459-1-APDownload protocol
IP protocol for ZIP8 antibody 20459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell

Regulation of the Catabolic Cascade in Osteoarthritis by the Zinc-ZIP8-MTF1 Axis.

Authors - Jin-Hong Kim
mouseIF

Nat Commun

The manganese transporter SLC39A8 links alkaline ceramidase 1 to inflammatory bowel disease

Authors - Eun-Kyung Choi
mouseWB,IF

J Clin Invest

Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity.

Authors - Wen Lin
  • KO Validated
mouseWB

Acta Neuropathol Commun

Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.

Authors - Alexandra Grubman
ratWB

Int J Mol Sci

Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn2+ Levels with Re-Distribution of Zn2+-Transporters in Aged Rat Cardiomyocytes.

Authors - Yusuf Olgar
mouseWB,IF

Int Immunopharmacol

ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx.

Authors - Gong Cheng
  • KD Validated
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