验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse heart tissue, U-937 cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human placenta tissue, mouse lung tissue, mouse small intestine tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20459-1-AP targets ZIP8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Immunogen | ZIP8 fusion protein Ag14292 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | solute carrier family 39 (zinc transporter), member 8 |
| Synonyms | BIGM103, PP3105, SLC39A8, ZIP 8, ZIP8 |
| Calculated Molecular Weight | 460 aa, 50 kDa |
| Observed Molecular Weight | 42-46 kDa, 75-90 kDa |
| GenBank Accession Number | BC012125 |
| Gene Symbol | ZIP8 |
| Gene ID (NCBI) | 64116 |
| RRID | AB_10697830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0K1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZIP8 antibody 20459-1-AP | Download protocol |
| IHC protocol for ZIP8 antibody 20459-1-AP | Download protocol |
| IP protocol for ZIP8 antibody 20459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The manganese transporter SLC39A8 links alkaline ceramidase 1 to inflammatory bowel disease | ||
J Clin Invest Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity.
| ||
Acta Neuropathol Commun Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder. | ||
Int J Mol Sci Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn2+ Levels with Re-Distribution of Zn2+-Transporters in Aged Rat Cardiomyocytes. | ||
Int Immunopharmacol ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx.
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