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SLC39A9 Polyclonal antibody

SLC39A9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17607-1-AP

Print datasheet

Synonyms

SLC39A9, Zinc transporter ZIP9, ZIP 9, ZIP9, Zrt and Irt like protein 9

验证数据展示

  • WB analysis using 17607-1-AP

    WB analysis using 17607-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17607-1-AP (SLC39A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 17607-1-AP

    IHC staining of human placenta using 17607-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17607-1-AP (SLC39A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHUVEC cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17607-1-AP targets SLC39A9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SLC39A9 fusion protein Ag11752 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 39 (zinc transporter), member 9
Synonyms SLC39A9, Zinc transporter ZIP9, ZIP 9, ZIP9, Zrt and Irt like protein 9
Calculated Molecular Weight 307 aa, 32 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC047682
Gene Symbol SLC39A9
Gene ID (NCBI) 55334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc transporter ZIP9 subfamily (SLC39A9) encodes a protein with characteristics of a membrane androgen receptor (mAR). Human ZIP9 is a 307-amino acid protein, which has eight putative transmembrane domains. ZIP9 has also been identified and characterized as a resident protein in the Golgi in DT40 and HeLa cell lines (PMID: 19420709, 23505453). ZIP9 may be present on the cell surface, as well as nuclear and mitochondrial membranes, in human breast and prostate cancer cell lines, where it mediates testosterone induction of apoptosis (PMID: 29952128). knockout of ZIP9 would lead to zinc accumulation in the secretory pathway, which could influence the general ion homeostasis and in turn the glycosylation outcome (PMID: 36648436).

实验方案

Product Specific Protocols
WB protocol for SLC39A9 antibody 17607-1-APDownload protocol
IHC protocol for SLC39A9 antibody 17607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols