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验证数据展示

  • WB analysis using 17607-1-AP

    WB analysis using 17607-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17607-1-AP (SLC39A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 17607-1-AP

    IHC staining of human placenta using 17607-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17607-1-AP (SLC39A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC39A9 Polyclonal antibody

SLC39A9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17607-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SLC39A9, Zinc transporter ZIP9, ZIP 9, ZIP9, Zrt and Irt like protein 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17607-1-AP targets SLC39A9 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 SLC39A9 fusion protein Ag11752 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 39 (zinc transporter), member 9
别名 SLC39A9, Zinc transporter ZIP9, ZIP 9, ZIP9, Zrt and Irt like protein 9
计算分子量 307 aa, 32 kDa
观测分子量25 kDa
GenBank蛋白编号BC047682
基因名称 SLC39A9
Gene ID (NCBI) 55334
RRIDAB_3085528
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUM3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc transporter ZIP9 subfamily (SLC39A9) encodes a protein with characteristics of a membrane androgen receptor (mAR). Human ZIP9 is a 307-amino acid protein, which has eight putative transmembrane domains. ZIP9 has also been identified and characterized as a resident protein in the Golgi in DT40 and HeLa cell lines (PMID: 19420709, 23505453). ZIP9 may be present on the cell surface, as well as nuclear and mitochondrial membranes, in human breast and prostate cancer cell lines, where it mediates testosterone induction of apoptosis (PMID: 29952128). knockout of ZIP9 would lead to zinc accumulation in the secretory pathway, which could influence the general ion homeostasis and in turn the glycosylation outcome (PMID: 36648436).

实验方案

Product Specific Protocols
WB protocol for SLC39A9 antibody 17607-1-APDownload protocol
IHC protocol for SLC39A9 antibody 17607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols