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验证数据展示

  • WB analysis of rat kidney using 84856-2-RR

    WB analysis of rat kidney using 84856-2-RR

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 84856-2-RR

    WB analysis of mouse kidney using 84856-2-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84856-2-RR

    Affinity and Kinetic Characterization of 84856-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84856-2-RR against Human SLC3A1 were performed. The affinity constant is below 1 pM.

SLC3A1 Recombinant antibody

SLC3A1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84856-2-RR

Print datasheet

CloneNo. 242370D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1, Amino acid transporter heavy chain SLC3A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat kidney tissue, mouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84856-2-RR targets SLC3A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC3A1 fusion protein Ag9525 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
Synonyms D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1, Amino acid transporter heavy chain SLC3A1
Calculated Molecular Weight 685 aa, 79 kDa
Observed Molecular Weight78-90 kDa
GenBank Accession NumberBC022386
Gene Symbol SLC3A1
Gene ID (NCBI) 6519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)

实验方案

Product Specific Protocols
WB protocol for SLC3A1 antibody 84856-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols