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SLC43A3 Polyclonal antibody

SLC43A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20131-1-AP

Print datasheet

Synonyms

Protein FOAP 13, PRO1659, FOAP 13, Equilibrative nucleobase transporter 1, ENBT1

验证数据展示

  • WB analysis using 20131-1-AP

    WB analysis using 20131-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20131-1-AP (SLC43A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 20131-1-AP

    IHC staining of human ovary cancer using 20131-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20131-1-AP (SLC43A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20131-1-AP targets SLC43A3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SLC43A3 fusion protein Ag13999 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 43, member 3
Synonyms Protein FOAP 13, PRO1659, FOAP 13, Equilibrative nucleobase transporter 1, ENBT1
Calculated Molecular Weight 491 aa, 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC003163
Gene Symbol SLC43A3
Gene ID (NCBI) 29015
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBI5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sodium-independent purine-selective nucleobase transporter(SLC43A3), also called as equilibrative nucleobase transporter 1(ENBT1), is a nucleobase transporter involved in purine salvage pathways in mammals, which mediates the equilibrative transport of extracellular purine nucleobases such as adenine, guanine and hypoxanthine.(PubMed:26455426, PubMed:32339528) It has been found that it may regulate the transport of fatty acids (FA) in fat cells, as a positive regulator of FA efflux and as a negative regulator of FA uptake.(PMID: 32217606)

实验方案

Product Specific Protocols
WB protocol for SLC43A3 antibody 20131-1-APDownload protocol
IHC protocol for SLC43A3 antibody 20131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols