验证数据展示
经过测试的应用
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human placenta tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:800-1:3200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81066-1-RR targets Band 3/AE 1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, IF-P, ELISA Application Description |
经测试反应性 | human |
免疫原 | Band 3/AE 1 fusion protein Ag27878 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) |
别名 | Band 3 / AE1, band 3, band 3/ AE1, SLC4A1, Anion exchanger 1 |
计算分子量 | 911 aa, 102 kDa |
GenBank蛋白编号 | BC101570 |
基因名称 | band 3 |
Gene ID (NCBI) | 6521 |
RRID | AB_2935547 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P02730 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for Band 3/AE 1 antibody 81066-1-RR | Download protocol |
IF protocol for Band 3/AE 1 antibody 81066-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |