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验证数据展示

  • WB analysis of mouse brain using 21848-1-AP

    WB analysis of mouse brain using 21848-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21848-1-AP (CHT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 21848-1-AP

    IHC staining of rat brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 21848-1-AP

    IHC staining of rat brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 21848-1-AP

    IP experiment of mouse heart using 21848-1-AP

    IP result of anti-CHT1 (IP:21848-1-AP, 4ug; Detection:21848-1-AP 1:500) with mouse heart tissue lysate 4000ug.

  • IF Staining of mouse brain using 21848-1-AP

    IF Staining of mouse brain using 21848-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CHT1 antibody (21848-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHT1 Polyclonal antibody

CHT1 Polyclonal Antibody for WB, IF-P, IP, ELISA
Cat No. 21848-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA and More (1)

Synonyms

SLC5A7, Hemicholinium-3-sensitive choline transporter, hCHT1, hCHT, CHT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse heart tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21848-1-AP targets CHT1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CHT1 fusion protein Ag16136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 5 (choline transporter), member 7
Synonyms SLC5A7, Hemicholinium-3-sensitive choline transporter, hCHT1, hCHT, CHT
Calculated Molecular Weight 580 aa, 63 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC111525
Gene Symbol CHT1
Gene ID (NCBI) 60482
RRIDAB_2878925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHT1 antibody 21848-1-APDownload protocol
IHC protocol for CHT1 antibody 21848-1-APDownload protocol
IF protocol for CHT1 antibody 21848-1-APDownload protocol
IP protocol for CHT1 antibody 21848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Mol Metab

Activation of a non-neuronal cholinergic system in visceral white adipose tissue of obese mice and humans

Authors - Ilenia Severi
View Article
mouseIHC

Adv Sci (Weinh)

CircFBXW4 Suppresses Colorectal Cancer Progression by Regulating the MiR-338-5p/SLC5A7 Axis

Authors - Wei Song
View Article