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验证数据展示

  • WB analysis using 29827-1-AP

    WB analysis using 29827-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29827-1-AP (SLC5A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 29827-1-AP

    IHC staining of rat brain using 29827-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC5A8 Polyclonal antibody

SLC5A8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29827-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Sodium-coupled monocarboxylate transporter 1, Sodium iodide-related cotransporter, SMCT1, SMCT, Apical iodide transporter

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29827-1-AP targets SLC5A8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC5A8 fusion protein Ag30183 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 5 (iodide transporter), member 8
别名 Sodium-coupled monocarboxylate transporter 1, Sodium iodide-related cotransporter, SMCT1, SMCT, Apical iodide transporter
计算分子量 610 aa, 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC110492
基因名称 SLC5A8
Gene ID (NCBI) 160728
RRIDAB_3086176
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 67-kDa protein that functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in healthy human, rat, and mouse colon. The protein is localized to the apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.

实验方案

Product Specific Protocols
WB protocol for SLC5A8 antibody 29827-1-APDownload protocol
IHC protocol for SLC5A8 antibody 29827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols