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SLC6A19 Polyclonal antibody

SLC6A19 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27575-1-AP

Print datasheet

Synonyms

Sodium-dependent neutral amino acid transporter B(0)AT1, HND, B0AT1

验证数据展示

  • IHC staining of human small intestine using 27575-1-AP

    IHC staining of human small intestine using 27575-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27575-1-AP (SLC6A19 antibody) at dilution of 1:400 (under 40x lens).

  • WB analysis using 27575-1-AP

    WB analysis using 27575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27575-1-AP (SLC6A19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 27575-1-AP

    WB analysis using 27575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27575-1-AP (SLC6A19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 27575-1-AP

    IHC staining of human small intestine using 27575-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27575-1-AP (SLC6A19 antibody) at dilution of 1:800 (under 10x lens).

  • IHC staining of human small intestine using 27575-1-AP

    IHC staining of human small intestine using 27575-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27575-1-AP (SLC6A19 antibody) at dilution of 1:800 (under 40x lens).

经过测试的应用

Positive WB detected inCaco-2 cells, mouse kidney tissue, PC-3 cells, mouse small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27575-1-AP targets SLC6A19 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC6A19 fusion protein Ag24362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 6 (neutral amino acid transporter), member 19
Synonyms Sodium-dependent neutral amino acid transporter B(0)AT1, HND, B0AT1
Calculated Molecular Weight 634 aa, 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC148801
Gene Symbol SLC6A19
Gene ID (NCBI) 340024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ695T7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC6A19, also named as B0AT1, encodes system B(0) transmembrane protein that mediates epithelial resorption of neutral amino acids across the apical membrane in kidney and intestine (PubMed: 15044460). The protein contains 634 amino acids and has 12 predicted transmembrane regions with highly expression in human kidney and small intestine and minimal in pancreas (PubMed: 15286787). The expression of SLC6A19 in intestinal cells depends on angiotensin converting enzyme 2 which is the cellular receptor for SARS-CoV-2 (PubMed: 32132184). Mutations in SLC6A19 cause Hartnup disease (PMID: 15286787, PMID: 15286788).

实验方案

Product Specific Protocols
WB protocol for SLC6A19 antibody 27575-1-APDownload protocol
IHC protocol for SLC6A19 antibody 27575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols