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验证数据展示

  • WB analysis using 30843-1-AP

    WB analysis using 30843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30843-1-AP (SLC6A20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 30843-1-AP

    IF Staining of mouse brain using 30843-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC6A20 antibody (30843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 30843-1-AP

    IF Staining of mouse brain using 30843-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC6A20 antibody (30843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC6A20 Polyclonal antibody

SLC6A20 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30843-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

XTRP3, XT3, Sodium/imino-acid transporter 1, Sodium- and chloride-dependent transporter XTRP3, SIT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30843-1-AP targets SLC6A20 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC6A20 fusion protein Ag33967 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 6 (proline IMINO transporter), member 20
别名 XTRP3, XT3, Sodium/imino-acid transporter 1, Sodium- and chloride-dependent transporter XTRP3, SIT1
观测分子量 50-60 kDa
GenBank蛋白编号BC136431
基因名称 SLC6A20
Gene ID (NCBI) 54716
RRIDAB_3669764
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NP91
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC6A20 also known as XT3, SIT1, belongs to the family of solute carrier 6, mediating the transport of neutral amino acids from the extracellular milieu toward cell or storage vesicles utilizing an electric membrane potential as the driving force. SLC6A20 is expressed in the kidney and small intestine and is localized in the brush marginal membrane of the proximal renal tubules (PMID: 16174864). Mutations in SLC6A20 are associated with Hyperglycinuria and Iminoglycinuria (PMID: 19033659)

实验方案

Product Specific Protocols
WB protocol for SLC6A20 antibody 30843-1-APDownload protocol
IF protocol for SLC6A20 antibody 30843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols