验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19559-1-AP targets Serotonin transporter in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 |
| 别名 | SLC6A4, SERT, HTT, hSERT, 5HTT |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa and 140 kDa |
| GenBank蛋白编号 | NM_001045 |
| 基因名称 | Serotonin transporter |
| Gene ID (NCBI) | 6532 |
| RRID | AB_2878590 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31645 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 kDa and 140 kDa bands in SDS-PAGE. The 140 kDa band corresponds to the dimer of high mannose-type SERT (PMID: 19323988).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Serotonin transporter antibody 19559-1-AP | Download protocol |
| IHC protocol for Serotonin transporter antibody 19559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress. | ||
Sci Rep Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability.
| ||
J Cell Physiol Serotonin Transporter Protects the Placental Cells Against Apoptosis in Caspase 3-Independent Pathway.
| ||
Heliyon Comparison of CUMS at different pregnancy stages, maternal separation, and their effects on offspring in postpartum depression mouse models | ||
Cell Rep SSRI antidepressant citalopram reverses the Warburg effect to inhibit hepatocellular carcinoma by directly targeting GLUT1 | ||
Eur J Neurosci The alteration of serotonergic markers in the amygdala and raphe nuclei of oestrogen receptor β knock-out female mice |
