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Serotonin transporter Polyclonal antibody

Serotonin transporter Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29186-1-AP

Print datasheet

Synonyms

5 HTT, 5 HTTLPR, 5HT transporter, 5HTT, hSERT, HTT, OCD1, serotonin transporter, SERT, SLC6A4

验证数据展示

  • WB analysis using 29186-1-AP

    WB analysis using 29186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29186-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29186-1-AP targets Serotonin transporter in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Serotonin transporter fusion protein Ag30412 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Synonyms 5 HTT, 5 HTTLPR, 5HT transporter, 5HTT, hSERT, HTT, OCD1, serotonin transporter, SERT, SLC6A4
Calculated Molecular Weight 70 aa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_001045
Gene Symbol Serotonin transporter
Gene ID (NCBI) 6532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319).

实验方案

Product Specific Protocols
WB protocol for Serotonin transporter antibody 29186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols