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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 29186-1-AP

    WB analysis using 29186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29186-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Serotonin transporter Polyclonal antibody

Serotonin transporter Polyclonal Antibody for WB, ELISA
Cat No. 29186-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, ELISA

别名

5 HTT, 5 HTTLPR, 5HT transporter, 5HTT, hSERT, HTT, OCD1, serotonin transporter, SERT, SLC6A4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, RAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29186-1-AP targets Serotonin transporter in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 Serotonin transporter fusion protein Ag30412 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
别名 5 HTT, 5 HTTLPR, 5HT transporter, 5HTT, hSERT, HTT, OCD1, serotonin transporter, SERT, SLC6A4
计算分子量 70 aa
观测分子量70 kDa
GenBank蛋白编号NM_001045
基因名称 Serotonin transporter
Gene ID (NCBI) 6532
RRIDAB_2935471
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319).

实验方案

Product Specific Protocols
WB protocol for Serotonin transporter antibody 29186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols