验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, Jurkat cells, HepG2 cells, rat brain/liver tissue, mouse heart tissue, rat liver tissue |
| Positive IHC detected in | human osteosarcoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14195-1-AP targets CAT-1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine |
| Immunogen | CAT-1 fusion protein Ag5398 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 |
| Synonyms | SLC7A1, ERR, CAT1 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 67-70 kDa |
| GenBank Accession Number | BC063303 |
| Gene Symbol | CAT-1 |
| Gene ID (NCBI) | 6541 |
| RRID | AB_2190723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30825 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAT-1 antibody 14195-1-AP | Download protocol |
| IHC protocol for CAT-1 antibody 14195-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.
| ||
J Cachexia Sarcopenia Muscle Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction. | ||
Free Radic Biol Med Cold plasma-treated medium preferentially eliminates doxorubicin-resistant osteosarcoma cells | ||
J Steroid Biochem Mol Biol High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1. | ||
Mol Cell Proteomics Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression. | ||
Front Oncol An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma. |
