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验证数据展示

  • WB analysis using 32384-1-AP

    WB analysis using 32384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32384-1-AP (SLC7A11/xCT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 32384-1-AP

    WB analysis using 32384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32384-1-AP (SLC7A11/xCT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse eye using 32384-1-AP

    IF Staining of mouse eye using 32384-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using SLC7A11/xCT antibody (32384-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC7A11/xCT Polyclonal antibody

SLC7A11/xCT Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 32384-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

sut, Subtle gray protein, Solute carrier family 7 member 11, Slc7a11, Cystine/glutamate transporter

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, HepG2 cells, rat brain tissue, mouse kidney tissue, mouse stomach tissue, rat liver tissue
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32384-1-AP targets SLC7A11/xCT in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC7A11/xCT fusion protein Ag38215 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
别名 sut, Subtle gray protein, Solute carrier family 7 member 11, Slc7a11, Cystine/glutamate transporter
计算分子量55 kDa
观测分子量70 kDa, 45 kDa
GenBank蛋白编号NM_011990
基因名称 Slc7a11
Gene ID (NCBI) 26570
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9WTR6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC7A11 (also known as xCT) is a membrane transporter involved in cysteine uptake. It promotes glutathione synthesis through cystine uptake and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers, including lung cancer. Although different from the predicted size of 55 kDa, the 45- and 70-kDa bands represent xCT, most likely a monomer and homodimer of the protein (PMID: 16399997).

实验方案

Product Specific Protocols
WB protocol for SLC7A11/xCT antibody 32384-1-APDownload protocol
IF protocol for SLC7A11/xCT antibody 32384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols