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SLC7A2 Polyclonal antibody

SLC7A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30232-1-AP

Print datasheet

Synonyms

HCAT2, Cationic amino acid transporter 2, CAT-2

验证数据展示

  • WB analysis using 30232-1-AP

    WB analysis using 30232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30232-1-AP (SLC7A2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 30232-1-AP

    IHC staining of human stomach cancer using 30232-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30232-1-AP (SLC7A2 antibody) at dilution of 1:160 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 30232-1-AP

    IHC staining of human stomach cancer using 30232-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30232-1-AP (SLC7A2 antibody) at dilution of 1:160 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30232-1-AP targets SLC7A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC7A2 fusion protein Ag31207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
Synonyms HCAT2, Cationic amino acid transporter 2, CAT-2
Calculated Molecular Weight 698 aa, 76 kDa
Observed Molecular Weight 76 kDa
GenBank Accession NumberBC104905
Gene Symbol SLC7A2
Gene ID (NCBI) 6542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Solute carrier family 7 member 2 (SLC7A2), also known as CAT2, is a member of the solute carrier superfamily. It functions as a cationic amino acid transporter, which can transport cationic amino acids (arginine, lysine and ornithine) into the cytosol and regulate inflammation (PMID: 35152203). It has been shown that lower SLC7A2 expression is associated with worse prognosis of ovarian cancer and hepatocellular carcinoma (PMID: 34108444; 32647070), and SLC7A2 deficiency in inflammatory bowel tissues increases the risk of inflammation-associated colon tumorigenesis (PMID: 30202097)

实验方案

Product Specific Protocols
WB protocol for SLC7A2 antibody 30232-1-APDownload protocol
IHC protocol for SLC7A2 antibody 30232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols