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验证数据展示

  • WB analysis of mouse brain using 15748-1-AP

    WB analysis of mouse brain using 15748-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15748-1-AP (SLC7A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15748-1-AP

    IHC staining of mouse brain using 15748-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15748-1-AP (SLC7A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15748-1-AP

    IF Staining of HeLa using 15748-1-AP

    Immunofluorescent analysis of Hela cells, using SLC7A4 antibody 15748-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SLC7A4 Polyclonal antibody

SLC7A4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15748-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

VH, HCAT3, CAT-4, CAT4, CAT 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, human liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15748-1-AP targets SLC7A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 SLC7A4 fusion protein Ag8211 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 7 (cationic amino acid transporter, y+ system), member 4
别名 VH, HCAT3, CAT-4, CAT4, CAT 4
计算分子量 635 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC008814
基因名称 SLC7A4
Gene ID (NCBI) 6545
RRIDAB_2190966
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43246
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC7A4 antibody 15748-1-APDownload protocol
IHC protocol for SLC7A4 antibody 15748-1-APDownload protocol
IF protocol for SLC7A4 antibody 15748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The Identification of the Metabolism Subtypes of Skin Cutaneous Melanoma Associated With the Tumor Microenvironment and the Immunotherapy.

Authors - Ronghua Yang
View Article