验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MCF-7 cells, HeLa cells, HepG2 cells, MKN-45 cells, SW480 cells |
| Positive IHC detected in | human placenta tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HT-29 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 11 publications below |
| IHC | See 3 publications below |
产品信息
28670-1-AP targets SLC7A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | SLC7A5 fusion protein Ag29164 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 |
| Synonyms | LAT1, D16S469E, CD98LC, CD98 light chain, 4F2LC |
| Calculated Molecular Weight | 507 aa, 55 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC039692 |
| Gene Symbol | SLC7A5 |
| Gene ID (NCBI) | 8140 |
| RRID | AB_2918188 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01650 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC7A5 antibody 28670-1-AP | Download protocol |
| IHC protocol for SLC7A5 antibody 28670-1-AP | Download protocol |
| IF protocol for SLC7A5 antibody 28670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Nucl Med Mol Imaging Synthesis, preclinical assessment, and first-in-human study of [18F]d4-FET for brain tumor imaging | ||
Mol Metab RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis | ||
J Inherit Metab Dis Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission | ||
Oncol Lett SLC7A5 regulates tryptophan uptake and PD‑L1 expression levels via the kynurenine pathway in ovarian cancer | ||
J Exp Clin Cancer Res CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex | ||
Front Oncol ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation |
