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验证数据展示

  • WB analysis using 30211-1-AP

    WB analysis using 30211-1-AP

    JAR cells were subjected to SDS PAGE followed by western blot with 30211-1-AP (SLC7A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 30211-1-AP

    WB analysis using 30211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30211-1-AP (SLC7A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 30211-1-AP

    IF Staining of HeLa using 30211-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SLC7A8 antibody (30211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC7A8 Polyclonal antibody

SLC7A8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30211-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Solute carrier family 7 member 8, LAT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, JAR cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30211-1-AP targets SLC7A8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SLC7A8 fusion protein Ag32957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
别名 Solute carrier family 7 member 8, LAT2
计算分子量58 kDa
观测分子量45-50 kDa
基因名称 SLC7A8
Gene ID (NCBI) 23428
RRIDAB_3086264
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHI5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Solute carrier family 7 member 8 (SLC7A8), also known as LAT2, is an amino acid transporter that, together with LAT1 (SLC7A5), facilitates the bidirectional transport of branched-chain and aromatic AAs across the plasma membrane.

实验方案

Product Specific Protocols
WB protocol for SLC7A8 antibody 30211-1-APDownload protocol
IF protocol for SLC7A8 antibody 30211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols