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SLC7A8 Polyclonal antibody

SLC7A8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30211-1-AP

Print datasheet

Synonyms

hLAT2, LAT2, LPI PC1, SLC7A8

验证数据展示

  • WB analysis using 30211-1-AP

    WB analysis using 30211-1-AP

    JAR cells were subjected to SDS PAGE followed by western blot with 30211-1-AP (SLC7A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 30211-1-AP

    WB analysis using 30211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30211-1-AP (SLC7A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 30211-1-AP

    IF Staining of HeLa using 30211-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SLC7A8 antibody (30211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, JAR cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30211-1-AP targets SLC7A8 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen SLC7A8 fusion protein Ag32957 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
Synonyms hLAT2, LAT2, LPI PC1, SLC7A8
Calculated Molecular Weight58 kDa
Observed Molecular Weight45-50 kDa
Gene Symbol SLC7A8
Gene ID (NCBI) 23428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHI5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC7A8 antibody 30211-1-APDownload protocol
IF protocol for SLC7A8 antibody 30211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols