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验证数据展示

  • WB analysis using 18891-1-AP

    WB analysis using 18891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 18891-1-AP

    IHC staining of human stomach cancer using 18891-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC9A3R2 Polyclonal antibody

SLC9A3R2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18891-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NHERF-2, NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18891-1-AP targets SLC9A3R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC9A3R2 fusion protein Ag13476 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
别名 NHERF-2, NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2
计算分子量 337 aa, 37 kDa
观测分子量45 kDa
GenBank蛋白编号BC106001
基因名称 SLC9A3R2
Gene ID (NCBI) 9351
RRIDAB_3085570
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15599
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)

实验方案

Product Specific Protocols
WB protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
IHC protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols