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SLC9A3R2 Polyclonal antibody

SLC9A3R2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18891-1-AP

Print datasheet

Synonyms

NHERF-2, NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2

验证数据展示

  • WB analysis using 18891-1-AP

    WB analysis using 18891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 18891-1-AP

    IHC staining of human stomach cancer using 18891-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18891-1-AP targets SLC9A3R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC9A3R2 fusion protein Ag13476 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
Synonyms NHERF-2, NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Calculated Molecular Weight 337 aa, 37 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC106001
Gene Symbol SLC9A3R2
Gene ID (NCBI) 9351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)

实验方案

Product Specific Protocols
WB protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
IHC protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols