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  • Featured Product
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NHE8 Polyclonal antibody

NHE8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18318-1-AP

Print datasheet

Synonyms

KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8

验证数据展示

  • WB analysis using 18318-1-AP

    WB analysis using 18318-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18318-1-AP

    WB analysis of HEK-293 using 18318-1-AP

    WB result of NHE8 antibody (18318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NHE8 transfected HEK-293 cells.

  • IHC staining of human kidney using 18318-1-AP

    IHC staining of human kidney using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 18318-1-AP

    IF Staining of HEK-293 using 18318-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18318-1-AP (NHE8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue, mouse colon tissue, mouse liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human colon tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18318-1-AP targets NHE8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NHE8 fusion protein Ag13111 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 8
Synonyms KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8
Calculated Molecular Weight 581 aa, 65 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC112213
Gene Symbol SLC9A8
Gene ID (NCBI) 23315
RRIDAB_2270442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NHE8, encoded by SLC9A8, is a member of sodium/hydrogen exchanger family which are transmembrane proteins that mediate the electroneutral exchange of sodium (Na) for hydrogen (H) and get involved in intracellular pH homeostasis, cell volume regulation, acid-base regulation, and so on. NHE8 has a broad tissue distribution, with relatively high abundance in the gastrointestinal tract. It has been reported that NHE8 expression differs among different regions in the stomach, very low in nonglandular region whereas very high in glandular region. The predicted molecular weight of NHE8 is 64-65 kDa, while its glycosylated form often migrates around 70-85 kDa.

实验方案

Product Specific Protocols
WB protocol for NHE8 antibody 18318-1-APDownload protocol
IHC protocol for NHE8 antibody 18318-1-APDownload protocol
IF protocol for NHE8 antibody 18318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols