验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human kidney tissue, human tonsillitis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
66577-1-Ig targets SLC9A9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Human, Mouse, Rat |
文献引用反应性 | human |
免疫原 | SLC9A9 fusion protein Ag4660 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | solute carrier family 9 (sodium/hydrogen exchanger), member 9 |
别名 | Na(+)/H(+) exchanger 9, Nbla00118, NHE 9, NHE9, SLC9A9, Sodium/hydrogen exchanger 9 |
计算分子量 | 645 aa, 73 kDa |
观测分子量 | 73 kDa |
GenBank蛋白编号 | BC035779 |
基因名称 | SLC9A9 |
Gene ID (NCBI) | 285195 |
RRID | AB_2881937 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q8IVB4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SLC9A9 antibody 66577-1-Ig | Download protocol |
IHC protocol for SLC9A9 antibody 66577-1-Ig | Download protocol |
IF protocol for SLC9A9 antibody 66577-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |